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Rochester New York
Orlando City B
 
Real Monarchs
Portland Timbers II
 
San Jose Earthquakes II
Houston Dynamo FC II
 
Columbus Crew II
Philadelphia Union II
 
Inter Miami II
Chicago Fire II
 
New York City II
FC Cincinnati II
 
Minnesota United II
Whitecaps II
 
Sporting Kansas City II
North Texas
 
St. Louis City II
Colorado Rapids II
 
Toronto II
New England II
 
Rochester New York
Philadelphia Union II
 
FC Cincinnati II
Chicago Fire II
 
New England II
New York City II
 
Orlando City B
Inter Miami II
 
Toronto II
Columbus Crew II
 
Colorado Rapids II
Sporting Kansas City II
 
Houston Dynamo FC II
Real Monarchs
 
North Texas
San Jose Earthquakes II
 
Minnesota United II
St. Louis City II
 
Portland Timbers II
Tacoma Defiance